Photoenzymatic Repair in Rainbow Trout (Oncorhynchus mykiss)
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In order to develop an understanding of the effects of increased UV radiation (UVR) reaching the Earth’s surface, it is important to investigate the abilities and limitations of organisms to repair damage caused by UVR. An important and well-studied mechanism for the repair of photoproducts, or DNA dimers caused by UVR radiation, is photoenzymatic repair (PER). The expression and rate of PER is known to vary greatly between species and between developmental stages within species. I used laboratory experiments to study the ontogeny, the effect of incubation light conditions, and the effect of UVR dose on the ability of rainbow trout (Oncorhynchus mykiss) to use PER as a significant form of DNA repair. Larvae were exposed to UVR radiation for 12 hours in the presence and absence of photorepair radiation. Survival was then monitored over a six day period in all treatments and in the last experiment, conducted at lower doses of UVR, frequencies of cyclobutane pyrimidine dimers were quantified using a radioimmunoassay analysis. Larvae were found to only display significant PER at lower doses of UVR exposure. There was no ontogenetic effect found for the expression of PER in rainbow trout and the effect of the presence of visible light during incubation is still unclear.
Franklin and Marshall College Archives, Undergraduate Honors Thesis 2007
- F&M Theses Collection