Thermostability of Phosphogluco-Isomerase with Regard to Coral Bleaching
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Coral bleaching is a phenomenon endangering some of the most diverse and complex marine ecosystems in the world. Bleaching is the result of corals’ expulsion of their symbiotic algae in response to sea surface temperature rise, among other stresses. This can cause the coral to be starved of valuable sugars that were provided through said symbionts. The reason for this expulsion not entirely clear, and it is the aim of this project to determine if differential enzyme thermostability between the corals and their symbionts is a plausible explanation. This project focused specifically on phosphoglucose isomerase (PGI), an important enzyme involved in glycolysis, and sought to compare its thermal stability in three different subject organisms: the coral Acropora millepora , a relatively thermotolerant algal symbiont Symbiodinium clade D, and a relatively thermosensitive algal symbiont Symbiodinium clade C. PGI from Acropora millepora displayed fairly high thermosensitivity, with a relatively low melting temperature when compared to another metabolic enzyme from the same organism. Comparisons between the coral and the two different clades of Symbiodinium were not possible because the complete PGI gene from either Symbiodinium C or Symbiodinium D could not be synthesized.
Franklin and Marshall College Archives, Undergraduate Honors Thesis 2018
- F&M Theses Collection