The extracellular protease AdamTS-B negatively regulates wing vein formation through BMP signaling in Drosophila melanogaster
Bone Morphogenetic Proteins (BMPs) are a group of secreted growth factors that orchestrate cellular behaviors during animal development. The wing of Drosophila melanogaster consists of two types of veins, longitudinal veins (L1-L6) and cross veins (posterior cross vein (PCV) and anterior cross vein (ACV)). Vein patterning in the Drosophila wing provides a powerful tool to study regulation of BMP signaling. Altering levels of BMP effectors can induce major defects in the cross veins and longitudinal veins of Drosophila wings. The extracellular protease AdamTS-B (also known as CG4096) encodes an ADAMTS protease expressed in the trachea and wing imaginal disc precursor cells of Drosophila embryos. AdamTS-B has been suggested to regulate wing venation, however, its regulatory mechanism remains unclear. Our hypothesis is that AdamTS-B controls wing vein formation by interacting with BMP effectors. In loss of AdamTS-B, the PCV had an extra vein formed, and the distal tip of the longitudinal L5 vein was branched or incomplete. These defects were similar to over-expression of BMP agonist crossveinless (cv), and down-regulation of the BMP inhibitor short gastrulation (sog). The defects observed in over-expression of AdamTS-B were also similar to those of sog up-regulation and cv knock-down mutations; PCV were not formed completely. Furthermore, the downstream effector of BMP signaling, pMad, showed altered expression levels consistent with the PCV defects seen in the loss and over-expression of AdamTS-B. These findings suggest that AdamTS-B might be a BMP antagonist, modifying BMP agonists or receptors to alter BMP signals. Further study needs to be undertaken to fully unravel interactions between AdamTS-B and BMP signaling pathway.
Franklin and Marshall College Archives, Undergraduate Honors Thesis 2015
- F&M Theses Collection